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Protocol for ABA-ELISA

download a pdf-file with the complete protocol for ABA determination through ELISA

  RIA and ELISA an Overview

See a short slide show about RIA and ELISA applications

  What is an Indirect ELISA

Schematic overview of the principle indirect Enzyme Linked Immuno Sorbent Assay

  ABA-ELISA evaluation

download an EXCEL spreadsheet for the analyses of crude extracts or xylem sap ABA-ELISA results

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Be aware that the option without or with reduced repetitions refers to the internal repetitions of the same sample on the plate

Iron detection in plant tissues

Hartmann, J., Asch, F. 2018.
Micro-method to determine iron concentrations in plant tissues using 2,2' bipyridine. Journal of Plant Nutrition and Soil Science, DOI: 10.1002/jpln.201700433.

Sample preparation for enzyme analyses

iHartmann, J., Asch, F. 2019.
Extraction, Storage Duration, and Storage Temperature Affect the Activity of Ascorbate Peroxidase, Glutathione Reductase, and Superoxide Dismutase in Rice Tissue. Biology 8, 70; doi:10.3390/biology8040070.

Extraction methods for K, Na and Cl determination

Asch, J., Johnson, K., Mondal, S., Asch, F. 2022.  Comprehensive assessment of extraction methods for plant tissue samples for determining sodium and potassium via flame photometer and chloride via automated flow analysis. Journal of Plant Nutrition and Soil Science, open access. https://doi.org/10.1002/jpln.202100344

Measuring whole plant transpiration in response to VPD

Pieters, A., Giese, M., Schmierer, M., Johnson, K., Asch, F. 2022.
Chamber-based system for measuring whole-plant transpiration dynamics. Plant-Environment Interactions, 00, 1 11. https://doi.org/10.1002/pei3.10094