Protocol
for ABA-ELISA |
download
a pdf-file with the complete protocol for ABA determination through
ELISA
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RIA
and ELISA an Overview |
See
a short slide show about RIA and ELISA applications
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What
is an Indirect ELISA |
Schematic
overview of the principle indirect Enzyme Linked Immuno Sorbent Assay
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ABA-ELISA
evaluation |
download
an EXCEL spreadsheet for the analyses of crude extracts or xylem sap
ABA-ELISA results
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Be aware that the option without or with reduced repetitions
refers to the internal repetitions of the same sample on the plate
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Iron detection in
plant tissues |
Hartmann, J., Asch, F.
2018.
Micro-method to determine iron concentrations in plant tissues using
2,2' bipyridine. Journal of Plant Nutrition and Soil Science, DOI: 10.1002/jpln.201700433. |
Sample preparation
for enzyme analyses |
iHartmann, J.,
Asch, F. 2019.
Extraction, Storage Duration, and Storage Temperature Affect the
Activity of Ascorbate Peroxidase, Glutathione Reductase, and Superoxide
Dismutase in Rice Tissue. Biology 8, 70; doi:10.3390/biology8040070. |
Extraction methods
for K, Na and Cl determination |
Asch, J., Johnson, K., Mondal, S., Asch,
F. 2022. Comprehensive assessment of extraction methods for
plant tissue samples for determining sodium and potassium via flame
photometer and chloride via automated flow analysis. Journal of Plant
Nutrition and Soil Science, open access.
https://doi.org/10.1002/jpln.202100344 |
Measuring whole
plant transpiration in response to VPD |
Pieters, A., Giese, M.,
Schmierer, M., Johnson, K., Asch, F.
2022.
Chamber-based system for measuring whole-plant transpiration dynamics.
Plant-Environment Interactions, 00, 1– 11.
https://doi.org/10.1002/pei3.10094 |